Pseudomonas aeruginosa PAO1, PA5076

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5076
Name
Replicon chromosome
Genomic location 5713232 - 5714032 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentStructural similarity to 5T0W (ancestral amino acid-binding protein AncCDT-1, root-mean-square deviation: 0.8 angstroms). Sequence similarity to 3K4U (96%/52,99%) (LYS). Final predicted ligand: L-amino acid (PMID: 31631799)

Cross-References

RefSeq NP_253763.1
GI 15600269
Affymetrix PA5076_at
Entrez 879765
GenBank AAG08461.1
INSDC AAG08461.1
NCBI Locus Tag PA5076
protein_id(GenBank) gb|AAG08461.1|AE004920_11|gnl|PseudoCAP|PA5076
TIGR NTL03PA05077
UniParc UPI00000C5EEB
UniProtKB Acc Q9HUA7
UniProtKB ID Q9HUA7_PSEAE
UniRef100 UniRef100_W0Z2L1
UniRef50 UniRef50_E2XL10
UniRef90 UniRef90_A0A086BTV7

Product

Feature Type CDS
Coding Frame 1
Product Name
putative amino acid ABC transporter substrate-binding protein
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 0.69
Kyte-Doolittle Hydrophobicity Value -0.141
Molecular Weight (kDa) 29.7
Isoelectric Point (pI) 7.58

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 2
Periplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 476 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004677 (454 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5076

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Uncovering the structure and function of Pseudomonas aeruginosa periplasmic proteins by an in silico approach.
Caprari S, Brandi V, Pasquadibisceglie A, Polticelli F
J Biomol Struct Dyn 2020 Sep;38(15):4508-4520
PubMed ID: 31631799