Pseudomonas aeruginosa PAO1, PA4403 (secA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4403
Name
secA
Replicon chromosome
Genomic location 4933866 - 4936616 (- strand)

Cross-References

RefSeq NP_253093.1
GI 15599599
Affymetrix PA4403_secA_at
Entrez 881307
GenBank AAG07791.1
INSDC AAG07791.1
NCBI Locus Tag PA4403
protein_id(GenBank) gb|AAG07791.1|AE004856_2|gnl|PseudoCAP|PA4403
TIGR NTL03PA04404
UniParc UPI00000C4ED9
UniProtKB Acc Q9LCT3
UniProtKB ID SECA_PSEAE
UniRef100 UniRef100_B7UZI1
UniRef50 UniRef50_P43803
UniRef90 UniRef90_A6VB75

Product

Feature Type CDS
Coding Frame 1
Product Name
secretion protein SecA
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected using LC-MS/MS (103.85 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -26.29
Kyte-Doolittle Hydrophobicity Value -0.505
Molecular Weight (kDa) 103.9
Isoelectric Point (pI) 5.35

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 588 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003993 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4403
Search term: secA

Human Homologs

References

Nucleotide sequence of the secA gene and secA(Ts) mutations preventing protein export in Escherichia coli.
Schmidt MG, Rollo EE, Grodberg J, Oliver DB
J Bacteriol 1988 Aug;170(8):3404-14
PubMed ID: 2841285
Cloning and expression of the secA gene of a marine bacterium, Vibrio alginolyticus, and analysis of its function in Escherichia coli.
Kunioka E, Matsuyama S, Tokuda H
Gene 1998 Aug 31;216(2):303-9
PubMed ID: 9729436
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344