| May 15, 2009 |
PA1998
|
Pseudomonas aeruginosa
PAO1 |
New gene name: dhcR. New product name: DhcR, transcriptional regulator. New product name confidence: Class 1. New comment: Deletion of dhcR or transposon insertions into the coding sequence result in a strain unable to grow using L-carnitine as a sole carbon source. DhcR regulates the transcriptional induction of the adjacent dhcAB genes in response to an L-carnitine catabolite predicted to be 3-dehydrocarnitine.. New evidence reference (PMID): 19406895.
|
Matthew J. Wargo (University of Vermont College of Medicine) and Deborah A. Hogan (Dartmouth Medical School)
|
| May 15, 2009 |
PA1999
|
Pseudomonas aeruginosa
PAO1 |
New gene name: dhcA. New product name: DhcA, dehydrocarnitine CoA transferase, subunit A. New product name confidence: Class 1. New comment: Deletion of dhcA or transposon insertions into the coding sequence result in a strain unable to grow using L-carnitine as a sole carbon source. DhcA is predicted to form a complex with DhcB which functions to deacetylate 3-dehydrocarnitine generating acetyl-CoA and glycine betaine CoA. Deletion of the dhcAB operon results in complete loss of carnitine-dependent CoA utilization from cytoplasmic extracts compared to cytoplasm from wild-type cells.; Transcription of the dhcAB operon is regulated by the transcription factor DhcR. . New evidence reference (PMID): 19406895. New genomic context: dhcAB operon.
|
Matthew J. Wargo (University of Vermont College of Medicine) and Deborah A. Hogan (Dartmouth Medical School)
|
| May 15, 2009 |
PA2000
|
Pseudomonas aeruginosa
PAO1 |
New gene name: dhcB. New product name: DhcB, dehydrocarnitine CoA transferase, subunit B. New product name confidence: Class 1. New comment: Deletion of dhcB or transposon insertions into the coding sequence result in a strain unable to grow using L-carnitine as a sole carbon source. DhcB is predicted to form a complex with DhcA which functions to deacetylate 3-dehydrocarnitine generating acetyl-CoA and glycine betaine CoA. Deletion of the dhcAB operon results in complete loss of carnitine-dependent CoA utilization from cytoplasmic extracts compared to cytoplasm from wild-type cells.;Transcription of the dhcAB operon is regulated by the transcription factor DhcR. . New evidence reference (PMID): 19406895. New genomic context: dhcAB operon.
|
Matthew J. Wargo (University of Vermont College of Medicine) and Deborah A. Hogan (Dartmouth Medical School)
|
| May 15, 2009 |
PA5385
|
Pseudomonas aeruginosa
PAO1 |
New gene name: cdhB . New product name: CdhB, Carnitine dehydrogenase-related gene B. New product name confidence: Class 1. New comment: Transposon insertion into cdhB results in a strain unable to grow using L-carnitine as a sole carbon source. The specific function of CdhB in carnitine catabolism is not known but bioinformatic analysis supports the strong conservation of the cdhCAB genes in all species known to aerobically catabolize L-carnitine.. New function class: Carbon compound catabolism. New evidence reference (PMID): 19406895.
|
Matthew J. Wargo (University of Vermont College of Medicine) and Deborah A. Hogan (Dartmouth Medical School)
|
| May 15, 2009 |
PA5386
|
Pseudomonas aeruginosa
PAO1 |
New gene name: cdhA. New product name: CdhA, Carnitine dehydrogenase. New product name confidence: Class 1. New comment: Deletion of cdhA or transposon insertion into the coding sequence results in a strain unable to grow using L-carnitine as a sole carbon source. Deletion of cdhCA results in complete loss of carnitine dehydrogenase activity as measured by carnitine-dependent NADH formation from cytoplasmic extracts.. New function class: Carbon compound catabolism. New evidence reference (PMID): 19406895.
|
Matthew J. Wargo (University of Vermont College of Medicine) and Deborah A. Hogan (Dartmouth Medical School)
|
| May 15, 2009 |
PA5387
|
Pseudomonas aeruginosa
PAO1 |
New gene name: cdhC. New product name: CdhC, Carnitine dehydrogenase-related gene C. New product name confidence: Class 1. New comment: Deletion of cdhC or transposon insertion into the coding sequence results in a strain unable to grow using L-carnitine as a sole carbon source. The specific function of CdhC in carnitine catabolism is not known but bioinformatic analysis supports the strong conservation of the cdhCAB genes in all species known to aerobically catabolize L-carnitine.. New function class: Carbon compound catabolism. New evidence reference (PMID): 19406895.
|
Matthew J. Wargo (University of Vermont College of Medicine) and Deborah A. Hogan (Dartmouth Medical School)
|
| May 15, 2009 |
PA5388
|
Pseudomonas aeruginosa
PAO1 |
New comment: Deletion of PA5388 or transposon insertion into the coding sequence results in a strain unable to grow using L-carnitine as a sole carbon source. Based on the requirement of this gene for growth, the PA5388 protein is predicted to be a carnitine-binding adapter for an ABC-family transporter.. New function class: Carbon compound catabolism. New evidence reference (PMID): 19406895.
|
Matthew J. Wargo (University of Vermont College of Medicine) and Deborah A. Hogan (Dartmouth Medical School)
|
| May 15, 2009 |
PA5389
|
Pseudomonas aeruginosa
PAO1 |
New gene name: cdhR. New product name: CdhR, transcriptional regulator. New product name confidence: Class 1. New comment: Deletion of cdhR results in a strain unable to grow using L-carnitine as a sole carbon source.;CdhR regulates the transcriptional induction of the adjacent PA5388 gene in response to L-carnitine. . New evidence reference (PMID): 19406895.
|
Matthew J. Wargo (University of Vermont College of Medicine) and Deborah A. Hogan (Dartmouth Medical School)
|