Pseudomonas aeruginosa PAO1, PA1999 (dhcA)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA1999
Name	dhcA Synonym: pcaI Synonym: atoD Synonym: scoA
Replicon	chromosome
Genomic location	2185527 - 2186225 (+ strand)
Transposon Mutants in orthologs	2 transposon mutants in orthologs
Comment	Deletion of dhcA or transposon insertions into the coding sequence result in a strain unable to grow using L-carnitine as a sole carbon source. DhcA is predicted to form a complex with DhcB which functions to deacetylate 3-dehydrocarnitine generating acetyl-CoA and glycine betaine CoA. Deletion of the dhcAB operon results in complete loss of carnitine-dependent CoA utilization from cytoplasmic extracts compared to cytoplasm from wild-type cells.; Transcription of the dhcAB operon is regulated by the transcription factor DhcR.

Cross-References

RefSeq	NP_250689.1
GI	15597195
Affymetrix	PA1999_at
DNASU	PaCD00006894
Entrez	878889
GenBank	AAG05387.1
INSDC	AAG05387.1
NCBI Locus Tag	PA1999
protein_id(GenBank)	gb|AAG05387.1|AE004626_6|gnl|PseudoCAP|PA1999
TIGR	NTL03PA01999
UniParc	UPI00000C554E
UniProtKB Acc	Q9I2B0
UniProtKB ID	Q9I2B0_PSEAE
UniRef100	UniRef100_W0YSZ8
UniRef50	UniRef50_P42315
UniRef90	UniRef90_A0A024HH44

Product

Feature Type	CDS
Coding Frame	1
Product Name	dehydrocarnitine CoA transferase, subunit A Product Name Confidence: Class 1
Synonyms	DhcA
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	-6.09
Kyte-Doolittle Hydrophobicity Value	-0.127
Molecular Weight (kDa)	25.2
Isoelectric Point (pI)	5.02

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543 Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 292 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG003436 (529 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA1999 Search term: dhcA Search term: dehydrocarnitine CoA transferase, subunit A

Human Homologs

Ensembl 110, assembly GRCh38.p14

ENSP00000196371 3-oxoacid CoA-transferase 1 [Source:HGNC Symbol;Acc:HGNC:8527] E-value: 3.0e-76 Percent Identity: 60.1

References

Succinyl CoA: 3-oxoacid CoA transferase (SCOT): human cDNA cloning, human chromosomal mapping to 5p13, and mutation detection in a SCOT-deficient patient. Kassovska-Bratinova S, Fukao T, Song XQ, Duncan AM, Chen HS, Robert MF, Pérez-Cerdá C, Ugarte M, Chartrand C, Vobecky S, Kondo N, Mitchell GA Am J Hum Genet 1996 Sep;59(3):519-28 PubMed ID: 8751852

Molecular characterization of lysR-lysXE, gcdR-gcdHG and amaR-amaAB operons for lysine export and catabolism: a comprehensive lysine catabolic network in Pseudomonas aeruginosa PAO1. Madhuri Indurthi S, Chou HT, Lu CD Microbiology (Reading) 2016 May;162(5):876-888 PubMed ID: 26967762