Pseudomonas putida NBRC 14164, PP4_09250 (pcaF)

Gene Feature Overview

Strain	Pseudomonas putida NBRC 14164 - Assembly GCF_000412675.1 GCF_000412675.1|latest
Locus Tag	PP4_09250
Name	pcaF
Replicon	chromosome
Genomic location	1063504 - 1064706 (+ strand)

Cross-References

RefSeq	YP_008111962.1
GI	512683982
Entrez	16138604
INSDC	BAN52778.1
UniParc	UPI00034F00B5
UniProtKB Acc	T2H2Y8
UniProtKB ID	T2H2Y8_PSEPU
UniRef100	UniRef100_T2H2Y8
UniRef50	UniRef50_P0C7L2
UniRef90	UniRef90_Q51956

Product

Feature Type	CDS
Coding Frame	1
Product Name	beta-ketoadipyl-CoA thiolase PcaF
Synonyms
Evidence for Translation
Charge (pH 7)	-3.90
Kyte-Doolittle Hydrophobicity Value	0.052
Molecular Weight (kDa)	41839.6
Isoelectric Point (pI)	5.77

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
6PCC	TRANSFERASE	06/17/19	Crystal structure of beta-ketoadipyl-CoA thiolase mutant (H356A) in complex hexanoyl coenzyme A	Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)	1.96	X-RAY DIFFRACTION	97.2
6PCB	TRANSFERASE	06/17/19	Crystal structure of beta-ketoadipyl-CoA thiolase mutant (H356A) in complex with COA	Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)	1.61	X-RAY DIFFRACTION	97.2
6PCD	TRANSFERASE	06/17/19	Crystal structure of beta-ketoadipyl-CoA thiolase mutant (C90S-H356A) in complex Octanoyl coenzyme A	Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)	1.37	X-RAY DIFFRACTION	97.0
6PCA	TRANSFERASE	06/17/19	Crystal structure of beta-ketoadipyl-CoA thiolase	Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)	1.81	X-RAY DIFFRACTION	97.5

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 483 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG000227 (529 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PP4_09250 Search term: pcaF Search term: beta-ketoadipyl-CoA thiolase PcaF

Human Homologs

Ensembl 110, assembly GRCh38.p14

ENSP00000285093 acetyl-CoA acyltransferase 2 [Source:HGNC Symbol;Acc:HGNC:83] E-value: 4.5e-80 Percent Identity: 43.2

References