Pseudomonas aeruginosa PAO1, PA5253 (algP)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5253
Name
algP
Synonym: algR3
Replicon chromosome
Genomic location 5915043 - 5916101 (- strand)
Transposon Mutants 3 transposon mutants in PAO1

Cross-References

RefSeq NP_253940.1
GI 15600446
Affymetrix PA5253_algP_at
DNASU PaCD00008264
Entrez 880806
GenBank AAG08638.1
INSDC AAG08638.1
NCBI Locus Tag PA5253
protein_id(GenBank) gb|AAG08638.1|AE004937_10|gnl|PseudoCAP|PA5253
TIGR NTL03PA05254
UniParc UPI0000125854
UniProtKB Acc P15276
UniProtKB ID ALGP_PSEAE
UniRef100 UniRef100_P15276
UniRef50 UniRef50_P15276
UniRef90 UniRef90_P15276

Product

Feature Type CDS
Coding Frame 1
Product Name
alginate regulatory protein AlgP
Product Name Confidence: Class 1
Synonyms Hp-1
transcription regulator AlgR3
Evidence for Translation
Detected using LC-MS/MS (34.49 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 55.91
Kyte-Doolittle Hydrophobicity Value -0.407
Molecular Weight (kDa) 34.5
Isoelectric Point (pI) 11.38

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Outer Membrane Vesicle Class 1
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database VFDB : VF0091
Category Alginate regulation
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 20 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004834 (122 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5253
Search term: algP

Human Homologs

References

DNA sequence and expression analysis of algP and algQ, components of the multigene system transcriptionally regulating mucoidy in Pseudomonas aeruginosa: algP contains multiple direct repeats.
Konyecsni WM, Deretic V
J Bacteriol 1990 May;172(5):2511-20
PubMed ID: 2110144
A procaryotic regulatory factor with a histone H1-like carboxy-terminal domain: clonal variation of repeats within algP, a gene involved in regulation of mucoidy in Pseudomonas aeruginosa.
Deretic V, Konyecsni WM
J Bacteriol 1990 Oct;172(10):5544-54
PubMed ID: 1698761
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
The histone-like protein AlgP regulon is distinct in mucoid and nonmucoid Pseudomonas aeruginosa and does not include alginate biosynthesis genes.
Cross AR, Csatary EE, Raghuram V, Diggle FL, Whiteley M, Wuest WM, Goldberg JB
Microbiology (Reading) 2020 Sep;166(9):861-866
PubMed ID: 32634088