Pseudomonas aeruginosa PAO1, PA4747 (secG)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4747
Name
secG
Replicon chromosome
Genomic location 5332354 - 5332743 (- strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253435.1
GI 15599941
Affymetrix PA4747_secG_at
Entrez 881707
GenBank AAG08133.1
INSDC AAG08133.1
NCBI Locus Tag PA4747
protein_id(GenBank) gb|AAG08133.1|AE004888_8|gnl|PseudoCAP|PA4747
TIGR NTL03PA04748
UniParc UPI00001357BE
UniProtKB Acc Q9HV52
UniProtKB ID SECG_PSEAE
UniRef100 UniRef100_Q9HV52
UniRef50 UniRef50_Q9HV52
UniRef90 UniRef90_Q9HV52

Product

Feature Type CDS
Coding Frame 1
Product Name
secretion protein SecG
Product Name Confidence: Class 2
Synonyms preprotein translocase band 1
Evidence for Translation
Detected using LC-MS/MS (13.21 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -3.29
Kyte-Doolittle Hydrophobicity Value 0.353
Molecular Weight (kDa) 13.2
Isoelectric Point (pI) 5.06

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 216 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004392 (395 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4747
Search term: secG

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
A novel membrane protein involved in protein translocation across the cytoplasmic membrane of Escherichia coli.
Nishiyama K, Mizushima S, Tokuda H
EMBO J 1993 Sep;12(9):3409-15
PubMed ID: 8253068
A new genetic selection identifies essential residues in SecG, a component of the Escherichia coli protein export machinery.
Bost S, Belin D
EMBO J 1995 Sep 15;14(18):4412-21
PubMed ID: 7556084
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344