Pseudomonas aeruginosa PAO1, PA4743 (rbfA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4743
Name
rbfA
Replicon chromosome
Genomic location 5326935 - 5327324 (- strand)
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253431.1
GI 15599937
Affymetrix PA4743_rbfA_at
DNASU PaCD00008059
Entrez 881688
GenBank AAG08129.1
INSDC AAG08129.1
NCBI Locus Tag PA4743
protein_id(GenBank) gb|AAG08129.1|AE004888_4|gnl|PseudoCAP|PA4743
TIGR NTL03PA04744
UniParc UPI00001331B8
UniProtKB Acc Q9HV56
UniProtKB ID RBFA_PSEAE
UniRef100 UniRef100_Q02FS9
UniRef50 UniRef50_Q4KIF5
UniRef90 UniRef90_Q02FS9

Product

Feature Type CDS
Coding Frame 1
Product Name
ribosome-binding factor A
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.05
Kyte-Doolittle Hydrophobicity Value -0.442
Molecular Weight (kDa) 14.5
Isoelectric Point (pI) 6.52

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 512 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004388 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4743
Search term: rbfA

Human Homologs

References

Suppression of a cold-sensitive mutation in 16S rRNA by overexpression of a novel ribosome-binding factor, RbfA.
Dammel CS, Noller HF
Genes Dev 1995 Mar 1;9(5):626-37
PubMed ID: 7535280
RbfA, a 30S ribosomal binding factor, is a cold-shock protein whose absence triggers the cold-shock response.
Jones PG, Inouye M
Mol Microbiol 1996 Sep;21(6):1207-18
PubMed ID: 8898389
RimM and RbfA are essential for efficient processing of 16S rRNA in Escherichia coli.
Bylund GO, Wipemo LC, Lundberg LA, Wikström PM
J Bacteriol 1998 Jan;180(1):73-82
PubMed ID: 9422595