Pseudomonas aeruginosa PAO1, PA5388 (caiX)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA5388
Name	caiX
Replicon	chromosome
Genomic location	6065856 - 6066794 (- strand)
Transposon Mutants	3 transposon mutants in PAO1
Comment	This gene has been experimentally demonstrated to be the carnitine transport periplasmic binding protein in P. aeruginosa.
Comment	Binds carnitine
Comment	Deletion of PA5388 or transposon insertion into the coding sequence results in a strain unable to grow using L-carnitine as a sole carbon source.

Cross-References

RefSeq	NP_254075.1
GI	15600581
Affymetrix	PA5388_at
Entrez	880509
GenBank	AAG08773.1
INSDC	AAG08773.1
NCBI Locus Tag	PA5388
protein_id(GenBank)	gb|AAG08773.1|AE004951_7|gnl|PseudoCAP|PA5388
TIGR	NTL03PA05389
UniParc	UPI00000C5FD9
UniProtKB Acc	Q9HTH6
UniProtKB ID	Q9HTH6_PSEAE
UniRef100	UniRef100_W0Z3J4
UniRef50	UniRef50_G8Q1M3
UniRef90	UniRef90_A6VEK0

Product

Feature Type	CDS
Coding Frame	1
Product Name	CaiX Product Name Confidence: Class 1
Synonyms	carnitine-specific binding protein
Evidence for Translation
Charge (pH 7)	3.38
Kyte-Doolittle Hydrophobicity Value	-0.081
Molecular Weight (kDa)	33.8
Isoelectric Point (pI)	8.75

Subcellular localization

Individual Mappings	Localization Confidence PMID Unknown Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 145 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG004947 (484 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA5388 Search term: caiX Search term: CaiX

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen. Lewenza S, Gardy JL, Brinkman FS, Hancock RE Genome Res 2005 Feb;15(2):321-9 PubMed ID: 15687295

The ATP-binding cassette transporter Cbc (choline/betaine/carnitine) recruits multiple substrate-binding proteins with strong specificity for distinct quaternary ammonium compounds. Chen C, Malek AA, Wargo MJ, Hogan DA, Beattie GA Mol Microbiol 2010 Jan;75(1):29-45 PubMed ID: 19919675