Pseudomonas aeruginosa PAO1, PA4942 (hflK)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4942
Name
hflK
Replicon chromosome
Genomic location 5545786 - 5546988 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_253629.1
GI 15600135
Affymetrix PA4942_hflK_at
Entrez 877755
GenBank AAG08327.1
INSDC AAG08327.1
NCBI Locus Tag PA4942
protein_id(GenBank) gb|AAG08327.1|AE004907_5|gnl|PseudoCAP|PA4942
TIGR NTL03PA04943
UniParc UPI00000C5E89
UniProtKB Acc Q9HUM2
UniProtKB ID Q9HUM2_PSEAE
UniRef100 UniRef100_W0YZ19
UniRef50 UniRef50_C5BRH5
UniRef90 UniRef90_W0YZ19

Product

Feature Type CDS
Coding Frame 1
Product Name
protease subunit HflK
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected using LC-MS/MS (44.04 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -7.74
Kyte-Doolittle Hydrophobicity Value -0.674
Molecular Weight (kDa) 44.0
Isoelectric Point (pI) 4.88

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 533 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004557 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4942
Search term: hflK

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Identification of polypeptides encoded by an Escherichia coli locus (hflA) that governs the lysis-lysogeny decision of bacteriophage lambda.
Banuett F, Herskowitz I
J Bacteriol 1987 Sep;169(9):4076-85
PubMed ID: 3040675
A protease complex in the Escherichia coli plasma membrane: HflKC (HflA) forms a complex with FtsH (HflB), regulating its proteolytic activity against SecY.
Kihara A, Akiyama Y, Ito K
EMBO J 1996 Nov 15;15(22):6122-31
PubMed ID: 8947034
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344