Pseudomonas aeruginosa PAO1, PA4843 (gcbA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Ontology

Ontology Accession Term GO Evidence Evidence Ontology (ECO) Code Reference Comments
Molecular Function GO:0009975 cyclase activity

Molecular Function GO:0052621 diguanylate cyclase activity
EXP
Inferred from Experiment
ECO:0000269
experimental evidence used in manual assertion
24603766 Reviewed by curator
Biological Process GO:1902065 response to L-glutamate
IMP
Inferred from Mutant Phenotype
ECO:0000016
loss-of-function mutant phenotype evidence
25331436 Reviewed by curator
Biological Process GO:1900233 positive regulation of single-species biofilm formation on inanimate substrate
IMP
Inferred from Mutant Phenotype
ECO:0000315
mutant phenotype evidence used in manual assertion
24603766 Reviewed by curator
Biological Process GO:1902201 negative regulation of bacterial-type flagellum-dependent cell motility
ISS
Inferred from Sequence or Structural Similarity
Term mapped from: UniProtKB:Q3KIN9
ECO:0000250
sequence similarity evidence used in manual assertion
24891445 Reviewed by curator
Molecular Function GO:0052621 diguanylate cyclase activity
IMP
Inferred from Mutant Phenotype
ECO:0000315
mutant phenotype evidence used in manual assertion
24603766 Reviewed by curator
Biological Process GO:1900189 positive regulation of cell adhesion involved in single-species biofilm formation
ISS
Inferred from Sequence or Structural Similarity
Term mapped from: UniProtKB:Q3KIN9
ECO:0000250
sequence similarity evidence used in manual assertion
24891445 Reviewed by curator
Molecular Function GO:0052621 diguanylate cyclase activity
IDA
Inferred from Direct Assay
ECO:0000005
enzyme assay evidence
24891445 Reviewed by curator
Biological Process GO:0000160 phosphorelay signal transduction system
ISM
Inferred from Sequence Model
Term mapped from: InterPro:PF00072
ECO:0000259
match to InterPro signature evidence used in automatic assertion

Functional Classifications Manually Assigned by PseudoCAP

Two-component regulatory systems Other PAO1 genes in this class
Transcriptional regulators Other PAO1 genes in this class

Pathways

Database Xref Pathway Version Evidence PMID
PseudoCAP Two-component System ECO:0000037
not_recorded

Functional Predictions from Interpro

Analysis Accession Description Interpro Accession Interpro Description Amino Acid Start Amino Acid Stop E-value
Gene3D G3DSA:3.30.70.270 - IPR043128 Reverse transcriptase/Diguanylate cyclase domain 376 540 8.7E-53
SUPERFAMILY SSF55073 Nucleotide cyclase IPR029787 Nucleotide cyclase 382 539 5.49E-48
FunFam G3DSA:3.40.50.2300:FF:000346 Diguanylate cyclase response regulator - - 247 375 3.8E-63
SMART SM00448 REC_2 IPR001789 Signal transduction response regulator, receiver domain 250 363 2.8E-26
PANTHER PTHR45138 REGULATORY COMPONENTS OF SENSORY TRANSDUCTION SYSTEM - - 64 539 3.8E-93
SUPERFAMILY SSF52172 CheY-like IPR011006 CheY-like superfamily 246 378 7.26E-30
Pfam PF00072 Response regulator receiver domain IPR001789 Signal transduction response regulator, receiver domain 252 363 1.4E-21
Coils Coil Coil - - 48 68 -
SMART SM00267 duf1_3 IPR000160 GGDEF domain 366 537 2.6E-55
Gene3D G3DSA:3.40.50.2300 - - - 245 375 3.5E-27
SUPERFAMILY SSF52172 CheY-like IPR011006 CheY-like superfamily 134 243 3.22E-5
NCBIfam TIGR00254 JCVI: diguanylate cyclase (GGDEF) domain IPR000160 GGDEF domain 374 536 7.1E-46
FunFam G3DSA:3.30.70.270:FF:000001 Diguanylate cyclase domain protein - - 372 538 8.4E-49
Pfam PF00990 Diguanylate cyclase, GGDEF domain IPR000160 GGDEF domain 379 533 2.9E-41
CDD cd01949 GGDEF IPR000160 GGDEF domain 379 534 8.25412E-59
CDD cd00156 REC - - 253 353 5.43678E-23
Gene3D G3DSA:3.40.50.2300 - - - 112 243 1.0E-5

Search for additional functional domains at the NCBI CDD database website. Go to this protein's amino acid sequence and follow the link.