Pseudomonas Project PRJNA238228
| Project Title | ChIP-seq study of sigma factors in Pseudomonas aeruginosa |
| Released | |
| Project Database | BioProject |
| Project Accession | PRJNA238228 |
| Organism Name | Pseudomonas aeruginosa |
| Project Description | Sigma factors are master regulators of bacterial transcription which direct gene expression of specific subsets of genes. In particular, alternative sigma factors are well-known to be key players of bacterial adaptation to changing environments. To elucidate the regulatory network of sigma factors in P. aeruginosa, an integrative approach including ChIP-seq of 11 polyhistidine-tag sigma factors was performed to define the primary regulon of each sigma factor. Overall design: Sigma factor genes were fused to a polyhistidine-tag and provided in trans. Under optimal conditions regarding sigma factor activity and induction of sigma factor expression, DNA-sigma factor interactions were conserved by formaldehyde treatment. Upon DNA fragmentation by sonication, the complexes were specifically immunoprecipitated by polyclonal anti-6X His-tag antibodies and the purified DNA was analyzed by Illumina sequencing. DNA enrichment to a control strain was calculated and used for peak calling within promoter region (-500,+100) to identify directly regulated genes/operons. |
| Publication Citation | Pubmed:25780925 |
| Sample Provider Pi Institution | Prof. Susanne Häussler, Molecular Bacteriology, Helmholtz Centre for Infection Research |
| Sample Provider Pi Email | denitsa.alamanova@googlemail.com |
External Links
Biosamples associated with this project
| Link | 34 BioSample(s) |