Pseudomonas aeruginosa MH27, PAMH27_RS29280

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown

Gene Feature Overview

Strain Pseudomonas aeruginosa MH27
GCF_000513235.1|latest
Locus Tag
PAMH27_RS29280
Name
Synonym: PAMH27_5634
Replicon contig_5
Genomic location 251238 - 252161 (+ strand)

Cross-References

RefSeq WP_003095589.1
GI 489186170

Product

Feature Type CDS
Coding Frame 1
Product Name
lipase
Synonyms
Evidence for Translation
Charge (pH 7) 4.31
Kyte-Doolittle Hydrophobicity Value -0.232
Molecular Weight (kDa) 33.6
Isoelectric Point (pI) 8.46

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
8D8W HYDROLASE 06/09/22 Crystal structure of ChoE with Ser38 adopting alternative conformations Pseudomonas aeruginosa 1.4 X-RAY DIFFRACTION 98.6
8D91 HYDROLASE 06/09/22 Crystal structure of ChoE in complex with acetate and tetraethylammonium (TEA) Pseudomonas aeruginosa 1.49 X-RAY DIFFRACTION 98.6
6UQZ HYDROLASE 10/21/19 Crystal structure of ChoE D285N mutant in complex with acetate and thiocholine Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.43 X-RAY DIFFRACTION 98.3
8D8Z HYDROLASE 06/09/22 Crystal structure of ChoE N147A mutant in complex with thiocholine and chloride Pseudomonas aeruginosa 1.38 X-RAY DIFFRACTION 98.3
6UQW HYDROLASE 10/21/19 Crystal structure of ChoE in complex with acetate and thiocholine Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.65 X-RAY DIFFRACTION 98.6
6UQY HYDROLASE 10/21/19 Crystal structure of ChoE H288N mutant in complex with acetylthiocholine Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.57 X-RAY DIFFRACTION 98.3
8D8X HYDROLASE 06/09/22 Crystal structure of ChoE in complex with acetate and thiocholine (crystal form 2) Pseudomonas aeruginosa 1.36 X-RAY DIFFRACTION 98.6
8D90 HYDROLASE 06/09/22 Crystal structure of ChoE N147A mutant in complex with bromide ions Pseudomonas aeruginosa 1.82 X-RAY DIFFRACTION 98.3
6UR1 HYDROLASE 10/21/19 Crystal structure of ChoE S38A mutant in complex with acetate and acetylthiocholine Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.42 X-RAY DIFFRACTION 98.3
8D8Y HYDROLASE 06/09/22 Crystal structure of ChoE N147A mutant in complex with acetylthiocholine Pseudomonas aeruginosa 1.54 X-RAY DIFFRACTION 98.3
6UQV HYDROLASE 10/21/19 Crystal structure of ChoE, a bacterial acetylcholinesterase from Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.35 X-RAY DIFFRACTION 98.6
6UR0 HYDROLASE 10/21/19 Crystal structure of ChoE D285N mutant acyl-enzyme Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.8 X-RAY DIFFRACTION 97.9
6UQX HYDROLASE 10/21/19 Crystal structure of ChoE in complex with propionylthiocholine Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.85 X-RAY DIFFRACTION 98.6

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004538 (814 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PAMH27_RS29280
Search term: lipase

Human Homologs

References

No references are associated with this feature.