Pseudomonas aeruginosa PAO1, PA5288 (glnK)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5288
Name
glnK
Replicon chromosome
Genomic location 5952483 - 5952821 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253975.1
GI 15600481
Affymetrix PA5288_glnK_at
DNASU PaCD00008286
Entrez 878342
GenBank AAG08673.1
INSDC AAG08673.1
NCBI Locus Tag PA5288
protein_id(GenBank) gb|AAG08673.1|AE004941_6|gnl|PseudoCAP|PA5288
TIGR NTL03PA05289
UniParc UPI00000D2F6F
UniProtKB Acc Q9HTR6
UniProtKB ID Q9HTR6_PSEAE
UniRef100 UniRef100_Q7BBU1
UniRef50 UniRef50_D4GKK7
UniRef90 UniRef90_Q7BBU1

Product

Feature Type CDS
Coding Frame 1
Product Name
nitrogen regulatory protein P-II 2
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.77
Kyte-Doolittle Hydrophobicity Value -0.054
Molecular Weight (kDa) 12.3
Isoelectric Point (pI) 5.22

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 489 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004865 (537 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5288
Search term: glnK

Human Homologs

References

Cloning and organization of the abc and mdl genes of Escherichia coli: relationship to eukaryotic multidrug resistance.
Allikmets R, Gerrard B, Court D, Dean M
Gene 1993 Dec 22;136(1-2):231-6
PubMed ID: 7904973
An alternative PII protein in the regulation of glutamine synthetase in Escherichia coli.
van Heeswijk WC, Hoving S, Molenaar D, Stegeman B, Kahn D, Westerhoff HV
Mol Microbiol 1996 Jul;21(1):133-46
PubMed ID: 8843440
Genetic determinants involved in the susceptibility of Pseudomonas aeruginosa to beta-lactam antibiotics.
Alvarez-Ortega C, Wiegand I, Olivares J, Hancock RE, Martínez JL
Antimicrob Agents Chemother 2010 Oct;54(10):4159-67
PubMed ID: 20679510