Pseudomonas aeruginosa PAO1, PA5176

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5176
Name
Synonym: yrfE
Replicon chromosome
Genomic location 5828904 - 5829470 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentLack of PA5176 NUDIX hydrolase increases bacterial resistance to killing by H2O2, however deletion of NUDIX hydrolases PA3470, PA3754, PA4400, and PA4841 causes sensitization to H2O2 killing [PMID:32419387]
CommentPA5176 mutants display attenuated virulence compared to the wild type in C. elegans infection model [PMID:32419387].

Cross-References

RefSeq NP_253863.1
GI 15600369
Affymetrix PA5176_at
Entrez 881740
GenBank AAG08561.1
INSDC AAG08561.1
NCBI Locus Tag PA5176
protein_id(GenBank) gb|AAG08561.1|AE004930_7|gnl|PseudoCAP|PA5176
TIGR NTL03PA05177
UniParc UPI00000C5F39
UniProtKB Acc Q9HU13
UniProtKB ID Q9HU13_PSEAE
UniRef100 UniRef100_Q9HU13
UniRef50 UniRef50_Q4KJZ0
UniRef90 UniRef90_M4XA08

Product

Feature Type CDS
Coding Frame 1
Product Name
NUDIX hydrolase
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -10.31
Kyte-Doolittle Hydrophobicity Value -0.257
Molecular Weight (kDa) 21.0
Isoelectric Point (pI) 4.52

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 428 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004764 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5176
Search term: NUDIX hydrolase

Human Homologs

References

Individual Nudix hydrolases affect diverse features of Pseudomonas aeruginosa.
Drabinska J, Ziecina M, Modzelan M, Jagura-Burdzy G, Kraszewska E
Microbiologyopen 2020 Aug;9(8):e1052
PubMed ID: 32419387