Pseudomonas aeruginosa PAO1, PA4954 (motA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4954
Name
motA
Synonym: rpmA
Replicon chromosome
Genomic location 5559021 - 5559872 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
CommentrpmA and rpmB gene products are required for efficient ingestion of P. aeruginosa by macrophages.

Cross-References

RefSeq NP_253641.1
GI 15600147
Affymetrix PA4954_motA_at
Entrez 879861
GenBank AAG08339.1
INSDC AAG08339.1
NCBI Locus Tag PA4954
protein_id(GenBank) gb|AAG08339.1|AE004908_5|gnl|PseudoCAP|PA4954
TIGR NTL03PA04955
UniParc UPI00000C5E91
UniProtKB Acc Q9HUL1
UniProtKB ID Q9HUL1_PSEAE
UniRef100 UniRef100_W0YY29
UniRef50 UniRef50_Q4KJ84
UniRef90 UniRef90_Q02F64

Product

Feature Type CDS
Coding Frame 1
Product Name
chemotaxis protein MotA
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -5.38
Kyte-Doolittle Hydrophobicity Value 0.449
Molecular Weight (kDa) 30.1
Isoelectric Point (pI) 5.61

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database VFDB
Category Flagella
Evidence ECO:0000250
sequence similarity evidence used in manual assertion
Host Organism Mus musculus
Notes rpmA (motA) null mutant was ingested by murine macrophages
Reference 10768936

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 259 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004569 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4954
Search term: motA

Human Homologs

References

RpmA is required for nonopsonic phagocytosis of Pseudomonas aeruginosa.
Simpson DA, Speert DP
Infect Immun 2000 May;68(5):2493-502
PubMed ID: 10768936
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Gene sequence and predicted amino acid sequence of the motA protein, a membrane-associated protein required for flagellar rotation in Escherichia coli.
Dean GE, Macnab RM, Stader J, Matsumura P, Burks C
J Bacteriol 1984 Sep;159(3):991-9
PubMed ID: 6090403
Membrane topology of the MotA protein of Escherichia coli.
Zhou J, Fazzio RT, Blair DF
J Mol Biol 1995 Aug 11;251(2):237-42
PubMed ID: 7643400