Pseudomonas aeruginosa PAO1, PA4941 (hflC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4941
Name
hflC
Replicon chromosome
Genomic location 5544917 - 5545786 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253628.1
GI 15600134
Affymetrix PA4941_hflC_at
DNASU PaCD00008139
Entrez 878721
GenBank AAG08326.1
INSDC AAG08326.1
NCBI Locus Tag PA4941
protein_id(GenBank) gb|AAG08326.1|AE004907_4|gnl|PseudoCAP|PA4941
TIGR NTL03PA04942
UniParc UPI00000C5E88
UniProtKB Acc Q9HUM3
UniProtKB ID Q9HUM3_PSEAE
UniRef100 UniRef100_W0Z0C1
UniRef50 UniRef50_W0Z0C1
UniRef90 UniRef90_W0Z0C1

Product

Feature Type CDS
Coding Frame 1
Product Name
protease subunit HflC
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected using LC-MS/MS (33.12 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 8.73
Kyte-Doolittle Hydrophobicity Value -0.463
Molecular Weight (kDa) 33.1
Isoelectric Point (pI) 9.95

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 533 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004556 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4941
Search term: hflC

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Identification of polypeptides encoded by an Escherichia coli locus (hflA) that governs the lysis-lysogeny decision of bacteriophage lambda.
Banuett F, Herskowitz I
J Bacteriol 1987 Sep;169(9):4076-85
PubMed ID: 3040675
A protease complex in the Escherichia coli plasma membrane: HflKC (HflA) forms a complex with FtsH (HflB), regulating its proteolytic activity against SecY.
Kihara A, Akiyama Y, Ito K
EMBO J 1996 Nov 15;15(22):6122-31
PubMed ID: 8947034
Translocation, folding, and stability of the HflKC complex with signal anchor topogenic sequences.
Kihara A, Ito K
J Biol Chem 1998 Nov 6;273(45):29770-5
PubMed ID: 9792691
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344