Pseudomonas aeruginosa PAO1, PA4632

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA4632
Name
Replicon	chromosome
Genomic location	5199503 - 5200324 (- strand)
Transposon Mutants	2 transposon mutants in PAO1
Transposon Mutants in orthologs	1 transposon mutants in orthologs
Comment	Structural similarity to 3C37: putative peptidase Zn-dependent from Geobacter sulfurreducens (root-mean-square deviation: 1.0 angstroms)

Cross-References

RefSeq	NP_253322.1
GI	15599828
Affymetrix	PA4632_at
DNASU	PaCD00008007
Entrez	881207
GenBank	AAG08020.1
INSDC	AAG08020.1
NCBI Locus Tag	PA4632
protein_id(GenBank)	gb|AAG08020.1|AE004877_7|gnl|PseudoCAP|PA4632
TIGR	NTL03PA04633
UniParc	UPI00000C5D9C
UniProtKB Acc	Q9HVF9
UniProtKB ID	Q9HVF9_PSEAE
UniRef100	UniRef100_W0Z034
UniRef50	UniRef50_Q4K6D0
UniRef90	UniRef90_A6VC25

Product

Feature Type	CDS
Coding Frame	1
Product Name	putative Zn-dependent peptidase Product Name Confidence: Class 3
Synonyms
Evidence for Translation	Detected using LC-MS/MS (29.16 kDa, PMID:21751344) Detected by MALDI-TOF/TOF (PMID:19333994). Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	1.13
Kyte-Doolittle Hydrophobicity Value	-0.223
Molecular Weight (kDa)	29.2
Isoelectric Point (pI)	7.62

Subcellular localization

Individual Mappings	Localization Confidence PMID Outer Membrane Vesicle Class 1 Unknown Class 3 20472543 Periplasmic Class 1 19333994
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 339 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG004284 (534 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA4632 Search term: putative Zn-dependent peptidase

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen. Lewenza S, Gardy JL, Brinkman FS, Hancock RE Genome Res 2005 Feb;15(2):321-9 PubMed ID: 15687295

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen. Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM Proteomics 2009 Apr;9(7):1901-15 PubMed ID: 19333994

Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa. Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS Proteomics 2011 Aug;11(16):3424-9 PubMed ID: 21751344

Uncovering the structure and function of Pseudomonas aeruginosa periplasmic proteins by an in silico approach. Caprari S, Brandi V, Pasquadibisceglie A, Polticelli F J Biomol Struct Dyn 2020 Sep;38(15):4508-4520 PubMed ID: 31631799