Pseudomonas aeruginosa PAO1, PA4385 (groEL)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4385
Name
groEL
Synonym: mopA
Replicon chromosome
Genomic location 4915481 - 4917124 (- strand)
CommentLocalication to outer membrane vesicle confirmed in three separate studies (PMID:22909304, PMID:21751344 and PMID:26303878).

Cross-References

RefSeq NP_253075.1
GI 15599581
Affymetrix PA4385_groEL_at
Entrez 881348
GenBank AAG07773.1
INSDC AAG07773.1
NCBI Locus Tag PA4385
protein_id(GenBank) gb|AAG07773.1|AE004854_9|gnl|PseudoCAP|PA4385
TIGR NTL03PA04386
UniParc UPI0000036A5F
UniProtKB Acc P30718
UniProtKB ID CH60_PSEAE
UniRef100 UniRef100_B7UZG3
UniRef50 UniRef50_O66200
UniRef90 UniRef90_O33500

Product

Feature Type CDS
Coding Frame 1
Product Name
GroEL protein
Product Name Confidence: Class 1
Synonyms 60 kD chaperonin
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Verified using two-dimensional gel electrophoresis, MALDI-TOF MS and peptide mass mapping(pI:5.0, molecular weight:57.1, PMID:21674802)
Detected using LC-MS/MS (57.09 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -13.50
Kyte-Doolittle Hydrophobicity Value 0.037
Molecular Weight (kDa) 57.1
Isoelectric Point (pI) 4.76

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 2
Cytoplasmic Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 666 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003976 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4385
Search term: groEL
Search term: GroEL protein

Human Homologs

Ensembl 110, assembly GRCh38.p14
heat shock protein family D (Hsp60) member 1 [Source:HGNC Symbol;Acc:HGNC:5261]
E-value: 3.8e-146
Percent Identity: 50.7
Ensembl 110, assembly GRCh38.p14
heat shock protein family D (Hsp60) member 1 [Source:HGNC Symbol;Acc:HGNC:5261]
E-value: 3.8e-146
Percent Identity: 50.7
Ensembl 110, assembly GRCh38.p14
heat shock protein family D (Hsp60) member 1 [Source:HGNC Symbol;Acc:HGNC:5261]
E-value: 3.8e-146
Percent Identity: 50.7
Ensembl 110, assembly GRCh38.p14
heat shock protein family D (Hsp60) member 1 [Source:HGNC Symbol;Acc:HGNC:5261]
E-value: 3.8e-146
Percent Identity: 50.7
Ensembl 110, assembly GRCh38.p14
heat shock protein family D (Hsp60) member 1 [Source:HGNC Symbol;Acc:HGNC:5261]
E-value: 3.8e-146
Percent Identity: 50.7
Ensembl 110, assembly GRCh38.p14
heat shock protein family D (Hsp60) member 1 [Source:HGNC Symbol;Acc:HGNC:5261]
E-value: 3.8e-146
Percent Identity: 50.7
Ensembl 110, assembly GRCh38.p14
heat shock protein family D (Hsp60) member 1 [Source:HGNC Symbol;Acc:HGNC:5261]
E-value: 3.8e-146
Percent Identity: 50.7
Ensembl 110, assembly GRCh38.p14
heat shock protein family D (Hsp60) member 1 [Source:HGNC Symbol;Acc:HGNC:5261]
E-value: 3.8e-146
Percent Identity: 50.7
Ensembl 110, assembly GRCh38.p14
heat shock protein family D (Hsp60) member 1 [Source:HGNC Symbol;Acc:HGNC:5261]
E-value: 3.8e-146
Percent Identity: 50.7

References

Cloning and sequencing of the genes coding for the 10- and 60-kDa heat shock proteins from Pseudomonas aeruginosa and mapping of a species-specific epitope.
Sipos A, Klocke M, Frosch M
Infect Immun 1991 Sep;59(9):3219-26
PubMed ID: 1715325
Cloning and nucleotide sequence comparison of the groE operon of Pseudomonas aeruginosa and Burkholderia cepacia.
Jensen P, Fomsgaard A, Høiby N, Hindersson P
APMIS 1995 Feb;103(2):113-23
PubMed ID: 7538307
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomics of the oxidative stress response induced by hydrogen peroxide and paraquat reveals a novel AhpC-like protein in Pseudomonas aeruginosa.
Hare NJ, Scott NE, Shin EH, Connolly AM, Larsen MR, Palmisano G, Cordwell SJ
Proteomics 2011 Aug;11(15):3056-69
PubMed ID: 21674802
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Identification of proteins associated with the Pseudomonas aeruginosa biofilm extracellular matrix.
Toyofuku M, Roschitzki B, Riedel K, Eberl L
J Proteome Res 2012 Oct 5;11(10):4906-15
PubMed ID: 22909304
Proteome Profiles of Outer Membrane Vesicles and Extracellular Matrix of Pseudomonas aeruginosa Biofilms.
Couto N, Schooling SR, Dutcher JR, Barber J
J Proteome Res 2015 Oct 2;14(10):4207-22
PubMed ID: 26303878