Pseudomonas aeruginosa PAO1, PA2448

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2448
Name
Replicon chromosome
Genomic location 2748378 - 2750120 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentStructural similarity to 3IGH: uncharacterized metal-dependent hydrolase from Pyrococcus horikoscii (root-mean-square deviation: 0.9 angstroms) Hydrolase

Cross-References

RefSeq NP_251138.1
GI 15597644
Affymetrix PA2448_at
Entrez 882921
GenBank AAG05836.1
INSDC AAG05836.1
NCBI Locus Tag PA2448
protein_id(GenBank) gb|AAG05836.1|AE004672_4|gnl|PseudoCAP|PA2448
TIGR NTL03PA02448
UniParc UPI00000C56DA
UniProtKB Acc Q9I134
UniProtKB ID Q9I134_PSEAE
UniRef100 UniRef100_Q9I134
UniRef50 UniRef50_A0A0B7DAU6
UniRef90 UniRef90_Q9I134

Product

Feature Type CDS
Coding Frame 1
Product Name
putative hydrolase
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -3.68
Kyte-Doolittle Hydrophobicity Value -0.204
Molecular Weight (kDa) 62.8
Isoelectric Point (pI) 6.61

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 1
Unknown (This protein may have multiple localization sites) Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 337 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002864 (827 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2448

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Uncovering the structure and function of Pseudomonas aeruginosa periplasmic proteins by an in silico approach.
Caprari S, Brandi V, Pasquadibisceglie A, Polticelli F
J Biomol Struct Dyn 2020 Sep;38(15):4508-4520
PubMed ID: 31631799