Pseudomonas aeruginosa PAO1, PA2146

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2146
Name
Synonym: yciG
Replicon chromosome
Genomic location 2361706 - 2361873 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
CommentPA2146 encodes a small protein that is prevalent in various Gram-negative and Gram-positive bacterial species and highly expressed in biofilms formed by P. aeruginosa laboratory and clinical isolates [PMID:35798749]

Cross-References

RefSeq NP_250836.1
GI 15597342
Affymetrix PA2146_i_at
DNASU PaCD00006948
Entrez 881691
GenBank AAG05534.1
INSDC AAG05534.1
NCBI Locus Tag PA2146
protein_id(GenBank) gb|AAG05534.1|AE004642_1|gnl|PseudoCAP|PA2146
TIGR NTL03PA02146
UniParc UPI00000C55D0
UniProtKB Acc Q9I1W9
UniProtKB ID Q9I1W9_PSEAE
UniRef100 UniRef100_W0YP99
UniRef50 UniRef50_A6V636
UniRef90 UniRef90_A6V636

Product

Feature Type CDS
Coding Frame 1
Product Name
conserved hypothetical protein
Product Name Confidence: Class 4
Synonyms
Evidence for Translation
Charge (pH 7) 5.46
Kyte-Doolittle Hydrophobicity Value -1.595
Molecular Weight (kDa) 5.6
Isoelectric Point (pI) 10.91

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database PseudoCAP
Category Not available / Not known
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Hordeum vulgare
Notes
Infection model Barley seed pathogenicity assay
Reference 21261818

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 27 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003327 (613 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2146

Human Homologs

References

Molecular evolution of the Escherichia coli chromosome. I. Analysis of structure and natural variation in a previously uncharacterized region between trp and tonB.
Stoltzfus A, Leslie JF, Milkman R
Genetics 1988 Oct;120(2):345-58
PubMed ID: 3058546
A previously uncharacterized gene, PA2146, contributes to biofilm formation and drug tolerance across the ɣ-Proteobacteria.
Kaleta MF, Petrova OE, Zampaloni C, Garcia-Alcalde F, Parker M, Sauer K
NPJ Biofilms Microbiomes 2022 Jul 7;8(1):54
PubMed ID: 35798749
PA2146 Gene Knockout Is Associated With Pseudomonas aeruginosa Pathogenicity in Macrophage and Host Immune Response.
She P, Liu Y, Luo Z, Chen L, Zhou L, Hussain Z, Wu Y
Front Cell Infect Microbiol 2020;10:559803
PubMed ID: 33134189