Pseudomonas aeruginosa PAO1, PA2065 (pcoA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2065
Name
pcoA
Synonym: copA
Replicon chromosome
Genomic location 2263079 - 2264977 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_250755.1
GI 15597261
Affymetrix PA2065_pcoA_at
Entrez 877718
GenBank AAG05453.1
INSDC AAG05453.1
NCBI Locus Tag PA2065
protein_id(GenBank) gb|AAG05453.1|AE004633_4|gnl|PseudoCAP|PA2065
TIGR NTL03PA02065
UniParc UPI00000C5582
UniProtKB Acc Q9I250
UniProtKB ID Q9I250_PSEAE
UniRef100 UniRef100_Q9I250
UniRef50 UniRef50_Q0C1I0
UniRef90 UniRef90_Q9I250

Product

Feature Type CDS
Coding Frame 1
Product Name
copper resistance protein A precursor
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected using LC-MS/MS (69.85 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -12.23
Kyte-Doolittle Hydrophobicity Value -0.413
Molecular Weight (kDa) 69.9
Isoelectric Point (pI) 6.08

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 2
Periplasmic Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 328 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003385 (483 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2065
Search term: pcoA

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Nucleotide sequence and organization of copper resistance genes from Pseudomonas syringae pv. tomato.
Mellano MA, Cooksey DA
J Bacteriol 1988 Jun;170(6):2879-83
PubMed ID: 3372485
Copper resistance in Pseudomonas syringae mediated by periplasmic and outer membrane proteins.
Cha JS, Cooksey DA
Proc Natl Acad Sci U S A 1991 Oct 15;88(20):8915-9
PubMed ID: 1924351
Molecular genetics and transport analysis of the copper-resistance determinant (pco) from Escherichia coli plasmid pRJ1004.
Brown NL, Barrett SR, Camakaris J, Lee BT, Rouch DA
Mol Microbiol 1995 Sep;17(6):1153-66
PubMed ID: 8594334
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344