Pseudomonas aeruginosa PAO1, PA1553 (ccoO1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1553
Name
ccoO1
Synonym: ccoO
Synonym: fixO
Replicon chromosome
Genomic location 1690701 - 1691312 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_250244.1
GI 15596750
Affymetrix PA1553_at
DNASU PaCD00006710
Entrez 883010
GenBank AAG04942.1
INSDC AAG04942.1
NCBI Locus Tag PA1553
protein_id(GenBank) gb|AAG04942.1|AE004583_7|gnl|PseudoCAP|PA1553
TIGR NTL03PA01554
UniParc UPI00000C53D7
UniProtKB Acc Q9I3G4
UniProtKB ID Q9I3G4_PSEAE
UniRef100 UniRef100_W0YMB1
UniRef50 UniRef50_Q4KFE4
UniRef90 UniRef90_Q4KFE4

Product

Feature Type CDS
Coding Frame 1
Product Name
Cytochrome c oxidase, cbb3-type, CcoO subunit
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (22.76 kDa, PMID:21751344)
Charge (pH 7) 2.38
Kyte-Doolittle Hydrophobicity Value -0.348
Molecular Weight (kDa) 22.8
Isoelectric Point (pI) 8.22

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 216 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003806 (921 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1553
Search term: ccoO1

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Functional analysis of the fixNOQP region of Azorhizobium caulinodans.
Mandon K, Kaminski PA, Elmerich C
J Bacteriol 1994 May;176(9):2560-8
PubMed ID: 8169204
Structural and functional analysis of aa3-type and cbb3-type cytochrome c oxidases of Paracoccus denitrificans reveals significant differences in proton-pump design.
de Gier JW, Schepper M, Reijnders WN, van Dyck SJ, Slotboom DJ, Warne A, Saraste M, Krab K, Finel M, Stouthamer AH, van Spanning RJ, van der Oost J
Mol Microbiol 1996 Jun;20(6):1247-60
PubMed ID: 8809776
Functional and regulatory analysis of the two copies of the fixNOQP operon of Rhizobium leguminosarum strain VF39.
Schlüter A, Patschkowski T, Quandt J, Selinger LB, Weidner S, Krämer M, Zhou L, Hynes MF, Priefer UB
Mol Plant Microbe Interact 1997 Jul;10(5):605-16
PubMed ID: 9204566
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344