Pseudomonas aeruginosa PAO1, PA2586 (gacA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2586
Name
gacA
Replicon chromosome
Genomic location 2925788 - 2926432 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_251276.1
GI 15597782
Affymetrix PA2586_gacA_at
Entrez 880582
GenBank AAG05974.1
INSDC AAG05974.1
NCBI Locus Tag PA2586
protein_id(GenBank) gb|AAG05974.1|AE004687_3|gnl|PseudoCAP|PA2586
TIGR NTL03PA02586
UniParc UPI000012AFCE
UniProtKB Acc Q51373
UniProtKB ID GACA_PSEAE
UniRef100 UniRef100_Q51373
UniRef50 UniRef50_Q51373
UniRef90 UniRef90_Q51373

Product

Feature Type CDS
Coding Frame 1
Product Name
response regulator GacA
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.19
Kyte-Doolittle Hydrophobicity Value 0.040
Molecular Weight (kDa) 23.6
Isoelectric Point (pI) 6.12

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database VFDB
Category GacS/GacA two-component system
Evidence
Host Organism
Notes
Reference
Source 2
Database PseudoCAP
Category GacS/GacA two-component system
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Bombyx mori
Notes gacA mutant strains exhibit reduced killing of silkworm.
Reference 15727838

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 606 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002960 (533 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2586
Search term: gacA

Human Homologs

References

The global activator GacA of Pseudomonas aeruginosa PAO positively controls the production of the autoinducer N-butyryl-homoserine lactone and the formation of the virulence factors pyocyanin, cyanide, and lipase.
Reimmann C, Beyeler M, Latifi A, Winteler H, Foglino M, Lazdunski A, Haas D
Mol Microbiol 1997 Apr;24(2):309-19
PubMed ID: 9159518